Maternal Haploids Are Preferentially Induced by CENH3-tailswap Transgenic Complementation in Maize
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Maternal Haploids Are Preferentially Induced by CENH3-tailswap Transgenic Complementation in Maize
Doubled haploid plants are invaluable breeding tools but many crop species are recalcitrant to available haploid induction techniques. To test if haploid inducer lines can be engineered into crops, CENH3 (-∕-) and CENH3:RNAi lines were complemented by AcGREEN-tailswap-CENH3 or AcGREEN-CENH3 transgenes. Haploid induction rates were determined following testcrosses to wild-type plants after indep...
متن کاملNew inducers of maternal haploids in maize
On the basis of the first inducer of maternal haploids in maize (Zea mays L.), Stock 6 (Coe, 1959), a number of new inducer lines have been created (Tyrnov et al, 1984; Lasharmes et al, 1988; Sarkar et al, 1994; Shatskaya et al, 1994; Chalyk, 1999; Rober et al, 2005). The inducers possess dominant anthocyanin marker genes allowing haploids to be identified at different stages (dry seeds, seedli...
متن کاملCenh3: An Emerging Player in Haploid Induction Technology
True-breeding lines are required for the development and production of crop varieties. In a classical breeding approach these lines are obtained through inbreeding, and often 7-9 generations of inbreeding is performed to achieve the desired level of homozygosity, over a period of several years. In contrast, the chromosomes of haploids can be doubled to produce true-breeding lines in a single ge...
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Haploids and doubled haploids are invaluable for basic genetic studies and in crop improvement. A novel method of haploid induction through genetic engineering of the Centromere Histone Protein gene, CENH3, has been demonstrated in Arabidopsis. The present study was undertaken to develop haploid inducer (HI) lines of Brassica juncea based on the principles elaborated in Arabidopsis. B. juncea w...
متن کاملA genetic analysis of the origin of maternal haploids in maize.
irst case of haploidy in higher plants was reported in Datura stramonium T $ : ~ ~ ~ ~ ~ ~ ~ et al. 1922). Subsequently, haploid sporophytes have been discovered in many plant species; STADLER and RANDOLPH first reported haploidy in maize (1929 unpublished, cited by RANDOLPH 1932). Numerous investigations have been conducted on the occurrence of spontaneous and induced haploids in a number of s...
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ژورنال
عنوان ژورنال: Frontiers in Plant Science
سال: 2016
ISSN: 1664-462X
DOI: 10.3389/fpls.2016.00414